Study on the Effect of Natural Plant Product on the Swarming Motility and Biofilm Formation of Pseudomonas aeruginosa

Haldar, Susmita (2022) Study on the Effect of Natural Plant Product on the Swarming Motility and Biofilm Formation of Pseudomonas aeruginosa. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Susmita Haldar (17MS060)) 17MS060_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (4MB)

Abstract

Antimicrobial resistance is an ever-growing concern, with Pseudomonas aeruginosa being under the spotlight as an opportunistic drug resistant bacteria which causes chronic infections. It is therefore important to find alternative treatments to tackle this pathogen. As bacterial swarming and biofilm formation contribute towards drug resistance, in this present study, we investigated the effect of plant leaf extracts of Lantana camara and Cinnamomum camphora on P. aeruginosa biofilms and swarming colonies. The leaf extracts were able to visibly inhibit the swarming of Pseudomonas colonies, as well as significantly increased the biofilm load. This inverse regulation between swarming and biofilm formation can be targeted and used to study alternative treatments against P. aeruginosa. It was also observed that glucose supplementation shows a similar inverse regulation wherein it promotes swarming motility and inhibits biofilm formation. Therefore, this may imply that the complex carbon metabolism pathway of P.aeruginosa is also a critical factor and can be targeted to alleviate P. aeruginosa infections.

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