Studies on Modulation of eDNA and Persister Cells in Pseudomonas aeruginosa HRW.1-S3 Under Antibiotic Stress

Ram, Rahul M. (2022) Studies on Modulation of eDNA and Persister Cells in Pseudomonas aeruginosa HRW.1-S3 Under Antibiotic Stress. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Rahul M Ram (17MS090)) 17MS090_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

In this study the effect of Gentamicin and Lantana camara Plant extract were tested on the viability and biofilm formation ability of Pseudomonas aeruginosa HRW.1-S3. Also we tried to look upon synergistic effect of antibiotic coupled with the plant extract. It was seen that biofilm load increased while planktonic cells density decreased with antibiotic treatment. Synergistic effect of Plant extract and antibiotic was seen to some extent. Amount of extracellular DNA present in biofilms across all conditions was investigated through live/dead cell analysis. And it was observed that live to dead cell ratio decreases under different treatment conditions. The present study also involved the comparison of the existence of persister cell population in biofilm in absence or presence of antibiotic and/or plant extract. It was observed later in the study a low percentage of population present inside biofilm exhibit persister like properties. Also we tried to inhibit growth of biofilm by increasing plant extract concentration, but observed a gradual increase in biofilm with increasing concentration of antibiotic. Morphological studies were done on biofilms from different antibiotic conditions and noticeable differences could be seen across all conditions.

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