Effect of Cellular Deadhesion on Endosomal Recycling in HeLa Cells

Mondal, Aritra (2022) Effect of Cellular Deadhesion on Endosomal Recycling in HeLa Cells. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Aritra Mondal (17MS168)) 17MS168_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (4MB)

Abstract

Regulation of membrane tension is crucial for the large-scale coordination of cell boundary dynamics. A bidirectional flow of lipids in the form of endosomes, between the plasma membrane and the endosomal pool helps to maintain this steady-state condition. However, cells undergoing mechanical processes like deadhesion often change their shape from a flat spread out state to a rounded shape, reducing the tension of the membrane. In response to this sudden change, cells respond by tweaking the rates of endocytosis and exocytosis, such that excess membrane can be moved away from the membrane and homeostasis is achieved. Previous studies have shown that the temporal evolution of membrane tension during deadhesion, might be oscillatory. In this study, we have tried to investigate the dynamics of this regulation of tension by the endocytic machinery, using fluorescence microscopy to quantify the intracellular population of the endosomes. We have also built a computational model of the endocytic recycling system, which can describe the relative membrane tension for different conditions.

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