Force Spectroscopy of Adhesion Molecules in Near Equilibrium Regime

Saravanan, Yogesh (2022) Force Spectroscopy of Adhesion Molecules in Near Equilibrium Regime. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Yogesh Saravanan (17MS178)) 17MS178_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (5MB)

Abstract

Single Molecule Force spectroscopy (SMFS) techniques provide dynamics molecular insights of interaction in biomolecules. Almost all SMFS approaches have low experimental throughput, which limits statistics. Acoustic Force Spectroscopy (AFS) is a novel high throughput SMFS technique that uses acoustic waves to simultaneously apply forces on hundreds of tethered bonds. In this project, we used the AFS to investigate the unbinding forces between streptavidin-biotin in near equilibrium conditions. The results were interpreted a stochastic model based of transition state theory which reveal a third outer energy barrier in the energy landscape of streptavidin-biotin, which is in agreement with predictions from the molecular dynamics simulation. Chapter 1 is a brief introduction to dynamic force spectroscopy in the near equilibrium. Chapter 2 will talks in detail the theoretical framework that is built to understand the dynamic force spectroscopy. Chapter 3 will explain in detail the experimental methodology of the acoustic force spectroscopy. Chapter 4 & 5 the results and conclusions that were obtained in work is be presented and discussed.

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