Effect of Cinnamomum camphora leaf extract on biofilm formation of Pseudomonas aeruginosa HRW.1-S3 strain

Marndi, Diptismita (2023) Effect of Cinnamomum camphora leaf extract on biofilm formation of Pseudomonas aeruginosa HRW.1-S3 strain. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Diptismita Marndi (16MS053)) Thesis_16MS053.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

An opportunistic human pathogen called Pseudomonas aeruginosa infects immunocompromised individuals with severe acute and chronic illnesses. It forms antibiotic-resistant biofilms, which is one reason it is so persistent in clinical settings. The term "biofilm" refers to a sessile assemblage of microorganisms that may adhere to either a living or non-living surface and are encased in an extracellular polymeric substance (EPS) matrix. In EPS, proteins, lipids, polysaccharides, and extracellular DNA (eDNA) are all present. Plant extracts may have good efficacy on their own or be sources of potent antibacterial chemicals that can work against pathogen planktonic and/or biofilms. The Lauraceae family includes the species Cinnamomum camphora, which is endemic to South Korea, China, and India as well as other places including Australia and the Himalayas. Due to its anti-bacterial and anti-inflammatory activity its extract has been used in this study to observe the effect. In this study effect of leaf extract is observed, with our model plant being Cinnamomum camphora. The effect of Cinnamomum camphora on the vitality and biofilm-forming abilities of Pseudomonas aeruginosa is investigated. The overall effect of the plant extract employed in this study is discussed. Extracellular DNA present in biofilms was also investigated. An ethanolic extract of Cinnamomum camphora was tested to determine its antibacterial activity and affect on the growth of Pseudomonas aeruginosa biofilms. In our investigation, we found that high extract concentrations reduced the planktonic population while increasing the load of the biofilm, and that this was accompanied by an increase in the generation of eDNA, exoproteins, and exopolysaccharides.

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