Versatile Binding Modes of Cationic Cyanostilbenes with DNA: B to Z Transition & Energy Transfer

Kumar, Rohit (2023) Versatile Binding Modes of Cationic Cyanostilbenes with DNA: B to Z Transition & Energy Transfer. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Rohit Kumar (18MS109)) Thesis_18MS109.pdf - Submitted Version Restricted to Repository staff only Download (5MB)

Abstract

The objective of this research is to create luminescent materials based on supramolecular assemblies in aqueous media. These luminescent materials are a series of extended α cyanostilbene derivatives with di/tetra positi ve charge(s) in their structures, and the lengths of the alkyl chains attached to the pyridinium end were varied to modulate their self assembly properties (s). In their aggregated/solid phases, the well known chromophores cyanosilbenes (CSs) commonly disp lay aggregation induced enhanced emission (AIEE) features. Utilizing the spectroscopic and photophysical properties of cyano stilbene based cationic chromophore complexes, we developed a novel strategy for the detection of groove binding mode in calf thymu s DNA. Alteration in the secondary structure of calf thymus DNA (from B DNA to Z DNA) is also induced at the higher ratio [r = NCSN/ctDNA] of the cyanostilbenes. Furthermore, the cyanostilbene derivatives with ctDNA were utilized to contrive Fȍster Resonan ce Energy transfer based on light harvesting systems in aqueous media. This thesis consists of two chapters. In Chapter 1 , the impact of aggregation on emission is explored, along with a brief description of molecular self assembly, especially in aqueous m edia. This chapter also discusses the design of organic chromophores, i.e., cyanostilbene derivatives, their synthetic procedures, and the characterization used to carry out the research. We also introduce DNA “the molecule of Life” as an anionic charged s caffold, DNA binding modes, and structural variants of DNA Chapter 2 discusses the detection of biologically significant analyte DNA utilizing cationic cyanostilbenes and DNA binding via multivalent interaction or groove binding. we discuss the use of DNA as a biomolecular scaffold and cationic cyanostilbene derivatives as energy donors to construct artificial light harvesting systems in aqueous media.

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