Assembling a cellulase cocktail and optimization of it’s secretion in E. coli for biomass degradation

Suksham, , (2023) Assembling a cellulase cocktail and optimization of it’s secretion in E. coli for biomass degradation. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS dissertation of Suksham (18MS182)) Thesis_18MS182.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

vThe effective conversion of cellulose into fermentable sugars is a prerequisite for producing biofuels from lignocellulosic biomass. Various cellulase enzymes combine to carry out this action, effectively breaking cellulose down. For the sustainable production of biofuels and other value-added products, the optimized secretion of the cellulase cocktail in E. coli can potentially lower the cost and complexity of cellulase production. In this study, a cellulase cocktail composed of endoglucanases, exoglucanases, and ꞵ-glucosidases was assembled together in E. coli. We added the secretory tag to each cellulase to secrete them in the extracellular media for direct saccharification. The cloning and pathway assembly was based on an adapted modular assembly method involving different assembly levels through the golden gate assembly method. This establishment of these genetic circuits will further allow us to study the synergistic expression of cellulase.

Actions (login required)

View Item