Screening and Understanding the effect of NSAIDs(non-steroidal anti-inammatory drugs) in border cell migration during Drosophila melanogaster oogenesis (Part A)

Kar, Swastik (2021) Screening and Understanding the effect of NSAIDs(non-steroidal anti-inammatory drugs) in border cell migration during Drosophila melanogaster oogenesis (Part A). Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Swastik Kar (16MS192)) 16MS192_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (4MB)

Abstract

Cell migration or movement is one of the major and central processes which a play a crucial role in the process of development in the metazoans. Moving Cells mediate tissue formation during embryonic development, potentiate gastrulation and, aid in morphogen- esis and, tissue repair,Movement of cells are also associated with in ammation and tissue damage. Unfortunately aberrant cell migration is linked to cancer metastasis. Given the wide implication of cellular movement in normal and pathological conditions, there is always a push to understand the molecular and genetic basis as how cells move. Our main focus of this study is based on the rationale that in ammatory signals from the damaged tissue site stimulates the directed movement of immune cells. As anti- in amma- tory drugs impede cell movement, we are screening the Non-steroidal anti-in ammatory Drugs (NSAIDs) that may block movement of border cells in Drosophila oogenesis. .Bor- der cell migration in Drosophila oogenesis is an excellent model system for studying group cell movement. Incidentally migrating border cells exhibit partial epithelial to mes- enchymal transition similar to that observed metastasizing solid tumors. We shortlisted four commonly used Non-steroidal Anti-in ammatory drugs namely Aspirin, Diclofenac, Paracetamol and Naproxen and calculated their IC50 value. Subsequently incubation of the Drosophila egg chambers in NSAIDs around their IC50 concentration of the four NSAIDs impeded Border cell movement. Of the four, Diclofenac and Paracetamol were more e�cient in blocking the movement of border cells.Further analysis indicated that treatment of egg chambers with NSAIDs resulted in higher number of border cells in the migratory cluster. In addition, one observed that the integrity of the migratory cluster has been a�ected coupled with lower level of Phospho-C-JUN. Overall, this study suggests that the NSAIDs impedes the movemen

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