Effect of Antibiotics on Structural and Molecular Dynamics of Biofilm Formation: Implication in Antibacterial Therapy

Kumar, Abhinash (2015) Effect of Antibiotics on Structural and Molecular Dynamics of Biofilm Formation: Implication in Antibacterial Therapy. Masters thesis, Indian Institute of science education and research Kolkata.

PDF (MS dissertation of Abhinash Kumar (13IP013)) Abhinash_Kumar-13IP013.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

Bacteria are continuously evolving new ways to avoid the effect of antibiotics and infections are becoming more intense. Ability to form biofilm is one of such ways which potentiates the microorganisms to establish infection and also to tolerate antibiotic treatment. Thus, bacterial biofilm cause chronic infections because they show increased tolerance to antibiotics and resist phagocytosis, as well as other components of the innate and the adaptive immune systems of a host. The microorganisms in biofilm live in a self-produced matrix of extracellular polymeric substances (EPS) that form their immediate environment.EPS are mainly constituted of polysaccharides, proteins, nucleic acids and lipids; they provide the mechanical stability as well as minimize effect of antibiotics. Pseudomonas sp. are Gram-negative bacteria, and when grown under sub-Minimum Inhibitory Concentration (sub-MIC) conditions of tetracycline, intense biofilm were formed on glass cover-slips in 24 well plates and the extent of biofilm formation, with respect to compactness and multi-layer structure, was observed to be Tetracycline concentration dependent. Studies on relative contributions of extracellular polymeric substances including eDNA in regulation of biofilm structure formation was also studied.

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