Expression and Characterization of a Non Diaphanous Formin Delphilin

Dutta, Priyanka (2015) Expression and Characterization of a Non Diaphanous Formin Delphilin. PhD thesis, Indian Institute of Science Education and Research Kolkata.

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Abstract

Actin is a major component of cytoskeleton which organizes the cell cytoplasm of eukaryotic cells. Formins are actin nucleator present in all eukaryotes, necessary for cytokinesis, cell migration, cell adhesion, cell polarity The entire details of formins in different cellular activities are yet to be studied. In this context we have carried out an expression analysis of different formins in different mammalian adult tissues and in different age specific tissues. Interestingly among these formins Delphilin, a non diaphanous related formin, is unique in having consecutive PDZ domains at its N-terminus and is ambiguously present only in cerebellar Purkinje cells in the post synaptic region. The expression profile of Delphilin reveals its presence in lung, heart, spleen, small intestine and ovary along with brain at different developmental stages. Actin nucleation activity of Delphilin is negligible compared to other formins. It inhibits actin filament elongation which may be important for maintenance and functioning of synapse. Inhibition of actin nucleation activity of Delphilin by Glu2δR is a kind of direct regulation of activity of FH2 domain of non diaphanous related formin Delphilin, as most of the diaphanous related formins are regulated by Rho GTPase.

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