Mechanical Manipulation of Single Cell

Kishan, . (2015) Mechanical Manipulation of Single Cell. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS dissertation of Kishan (09MS16)) kishan09ms16.pdf - Submitted Version Restricted to Repository staff only Download (8MB)

Abstract

By Mechanical manipulation of cells, we can study cell's dynamics and responses in different mechanical conditions. A novel stretching device for imaging live-cell under strain is described in this project. The device could assemble onto a standard inverted microscopes and apply constant stretch or cyclic stretch of cells cultured on an elastic substrate or on a glass surface. The device is motion compensated to dynamically stretch cell on the stretchable substrate .This method keep the observed cell in the Field of View of microscope .The stretchable substrate used for this project is Polydimethylsiloxane (PDMS).Different properties of PDMS are characterized including the localized strains at different positions , effect of boundary shapes of membrane on localized strain,Force Versus Elongation and dependence of thickness on angular speed of spin coater . The other method used for mechanical manipulation uses edge of two coverslips controlled by peizomotors to apply cyclic or constant stretch of cells.The glass base in this method, provides an optically clear medium for live-cell imaging through inverted microscope.It does not require motion compensation as the length of maximum stretch lies within the field of view of microscope. These devices could be used to determine a measure of the membrane tension ensemble for a cell by pulling out tethers from multiple points.This is done by stretching cells on a specifically micro-patterned PDMS membrane.

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