IISER Kolkata ePrints Repository

Role of Histone Methyltransferases during Regeneration of Hydra

Pal, Mrinmoy (2018) Role of Histone Methyltransferases during Regeneration of Hydra. Masters thesis, Indian Institute of Science Education and Research Kolkata.

[img] PDF (MS dissertation of Mrinmoy Pal (13MS116)) - Submitted Version
Restricted to Repository staff only

Download (1375Kb)

    Abstract

    Hydra, a freshwater polyp of the phylum Cnidaria, has an immense ability to regenerate lost body parts by reorganizing the available tissue without involving cellular proliferation by a process known as morphallaxis. Evoking gene regulatory networks at precise time points is essential to achieve this feat. Epigenetic regulators presumably play the key role in fine-tuning these transcriptional changes by modulating the chromatin topography. However, the role of epigenetic modifiers in shaping the regeneration process in Hydra remains an open question. In this study, we attempted to characterize a number of histone methyltransferases from Hydra and investigated their role during regeneration. Preliminary screening performed using specific pharmacological inhibitors indicated a role of SETD8, an enzyme responsible for deposition of H4K20me1, during Hydra regeneration. Our work demonstrates a significant delay in the tentacle bud emergence and impairment of foot regeneration upon treating Hydra with UNC0379, a substrate-competitive inhibitor of SETD8, in the respective regeneration assays. Interestingly, SETD8 gets transiently upregulated in the regenerating tip during the early time points, i.e. 2 to 8 hours post amputation. Furthermore, ectopic activation of canonical Wnt signaling pathway, which has been well characterized for its role in head organizer function, leads to transcriptional upregulation of SETD8. Taken together, the findings from our study have led to the identification of a critical role played by SETD8 in the morphallactic regeneration of Hydra. Further investigation addressing the role of SETD8 in modulating the target gene regulation by H4K20me1 promises to provide novel insights into the epigenetic control of regeneration process.

    Item Type: Thesis (Masters)
    Additional Information: Supervisors: Prof. Dr. Sanjeev Galande and Dr. Tapas Kumar Sengupta
    Uncontrolled Keywords: Epigenetic Regulators; Histone Methyltransferases; Hydra; Phylum Cnidaria; SETD8
    Subjects: Q Science > QH Natural history > QH301 Biology
    Divisions: Department of Biological Sciences
    Depositing User: IISER Kolkata Librarian
    Date Deposited: 05 Dec 2018 12:40
    Last Modified: 05 Dec 2018 12:40
    URI: http://eprints.iiserkol.ac.in/id/eprint/735

    Actions (login required)

    View Item