Mining Laccase Sequences in Lignin Degrading Bacteria

Mutukula, Naresh (2013) Mining Laccase Sequences in Lignin Degrading Bacteria. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS Dissertation of Naresh Mutukula (11IP07)) Naresh_Mutukula-11IP07-MSThesis.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

Laccases are oxido-reductases which are widely spread in plants, insects, fungi and bacteria. We have redefined the signature pattern of laccase, by the addition of newly found signature patterns to the classical signatures reported earlier. Using this redefined signatures, searched the protein database to mine out new laccase sequences, among them have found some lignin degrading bacterial species with laccase sequences, important for the degradation of lignin part of lignocellulose biomass used for biofuel production. Laccases have varying redox potential capability of its T1 copper ranging from 790 mV to 430 mV. Explored the secondary sphere around T1 copper of high redox potential basidiomycete’s laccase, medium redox potential ascomycete’s laccase and low redox potential bacterial laccase; reported the conserved and diverged amino acids in the secondary sphere, as an attempt to find out the reason for varying redox potential nature of laccase.

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