Studies on the Role Played by Ribosome During Cold Stress in Bacteria

Sarkar, Parijat (2013) Studies on the Role Played by Ribosome During Cold Stress in Bacteria. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS dissertation of Parijat Sarkar) Parijat_Sarkar-08MS17-MSThesis.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

The objective of this project was to find out how ribosomes are involved in the cold-shock response. Upon cold-shock cells went for a growth arrest for 4 hours during which a major cold-shock protein was induced .In E. coli MRE 600 cells, upon cold-shock there was a lag phase of 4 hours (acclimatization phase) after which the microbe again started growing till saturation. During cold-shock period, expression of major cold-shock protein was observed in these cells. Till 6 hours of cold-shock the expression of the protein (suspected to be CspA on the basis of molecular weight) was observed in these cells. It is well known that the minimum growth temperature of this microbe is 10⁰C, so when cold-shock treatment was done at 15⁰C, the previously induced protein at 10⁰C was not induced to a very significant level. But for another enteropathogenic bacteria, Vibrio cholera N16961 the expression of this major cold-shock protein was found during the cold-shock temperature 15⁰C (data not shown).

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