Transcriptomic meta-analysis of Sepsis and other related illness

Choudhury, Shubham (2019) Transcriptomic meta-analysis of Sepsis and other related illness. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS dissertation of Shubham Choudhury (14MS026)) 14MS026.pdf - Submitted Version Restricted to Repository staff only Download (794kB)

Abstract

Sepsis is one of the leading diseases associated with high mortality and morbidity, due to the systemic nature of this illness, transcriptomic technology is particularly suited to the investigation of the molecular underpinning of survival from sepsis episodes. Osteoclast differentiation pathway was significantly up-regulated in septic shock patients compared to control. By performing pathway analysis, it was established that the pathway was also up-regulated in other conditions (sepsis, systemic inflammatory response syndrome (SIRS), critical illness, and bacterial infection). Visual verification was done using a heat map of the log fold changes of gene expression in all the studies. Certain genes of the osteoclast differentiation pathway were found to be associated with the survival of patients. These genes were checked for their significance of the association, and only the significant genes were analyzed further. Most of these genes were evidently up-regulated in survivors than in non-survivors. Temporal expression of these 15 genes was consistent with a role in survival from sepsis.

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