Graphene oxide (GO) modulates the aggregation kinetics of ⍺-synuclein

Kapoor, Rajat (2019) Graphene oxide (GO) modulates the aggregation kinetics of ⍺-synuclein. Masters thesis, Indian Institute of Science Education and Research Kolkata.

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Abstract

⍺-synuclein is a 14 kDa presynaptic neuronal protein which when aggregates is responsible for the second most prevalent neurodegenerative disorder known as Parkinson Disease. Inhibiting the ⍺-synuclein from getting aggregate is one of the most essential strategy to treat Parkinson Disease. Graphene is a remarkable material that is quickly changing the world around us. Graphene oxide have low-cytotoxicity and great biocompatibility which gives it edge over other carbon based material. Also there are reports which shows that Graphene and its compounds inhibits the aggregation of peptides and proteins. In addition to that the GO have high blood{brain barrier (BBB) permeability because of its small size. In this report, we have shown that GO can affect or modulate the aggregation kinetics of ⍺-synuclein and interact directly with mature fibrils by using various spectroscopic techniques such as Fluorescence, Circular Dichorism (CD), Atomic Force Microscopy (AFM) and Nuclear magnetic resonance (NMR) for residue specific interaction. The results obtained from all these, supports the possibility that a graphene based material with appropriate modifications might be developed to inhibit abnormal protein aggregation and amyloidosis.

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