Dissecting the dual specific nature of dyrk kinase

Gupta, Himanshu (2025) Dissecting the dual specific nature of dyrk kinase. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Himanshu Gupta (20MS188)) 20MS188_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

The tyrosine kinase signalling has co-evolved with multicellularity or, in fact, has played a key role in its development. How the evolution happened is still unclear. Are they direct descendants from the src module considered an ancient kinase fold for tyrosine signalling, or have they evolved from the dual specific kinase? Since the dual specific kinase is found along with serine/threonine kinase in lower organisms where the tyrosine kinase is absent, and they contain tyrosine signalling in its autophosphorylation activation, but they phosphorylate on serine/threonine residues on its substrate. So, it can be speculated that dual-specific kinases are the ancestors of tyrosine kinase and have descended from serine/threonine kinase. Along with this, dual specific kinase has evolved from lower organisms to higher organisms (Yak1 in yeast to Mnb in Drosophila to Dyrk1A in humans) functional divergence has taken place since it played a major role in yeast in negatively regulating cell cycle whereas in humans it is involved in wide range of processes like differentiation, fear learning and memory, neurodegeneration etc., what are the residues responsible for this divergence understanding by autophosphorylation nature of the three kinase here. After delineating the residues for making dual specific kinase into bonafide tyrosine kinase or serine/threonine kinase, it should be validated through mass spectrometry and site-directed mutation to know the importance of these residues.

Actions (login required)

View Item