Self-assembling Lipid Nanoparticles For Dendritic Cell-Based DNA Vaccination

Mukherjee, Swapnil (2021) Self-assembling Lipid Nanoparticles For Dendritic Cell-Based DNA Vaccination. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Swapnil Mukherjee (16MS001)) 16MS001_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

With the advent of SARS-Cov-2, efficient and fast production of vaccines have become a necessity. Genetic material-based vaccines have shown remarkable reliability in this situation, considering the ease of production and high immune response. DNA vaccines have become one of the most popular methods of vaccination in this context. They have found use in therapies for a wide range of diseases including cancer immunotherapy and gene therapy of rare diseases. But these require equally reliable carriers to ensure proper delivery which also provides adequate immune response. While liposomal nanoparticles have remained a popular method of delivery of these vaccines, their production is highly energy demanding and lack reproducibility. Which is why, I have attempted to synthesize a self-assembling single chained lipid nanoparticles which can bypass the issues faced during preparation of liposomes. In addition to being self assembling, these lipid nanoparticles have been hypothesized to target dendritic cells which are the most potent antigen presenting cells inside the body. This makes these lipids a highly attractive option for the delivery of DNA and other gene-based vaccines.

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