Lithocholic acid based metallogel formation and its enzymatic activity

Roy, Sampurna (2025) Lithocholic acid based metallogel formation and its enzymatic activity. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Sampurna Roy (20MS022)) 20MS022_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

Bile acids are produced from cholesterol in liver and are subsequently released into the intestinal tract to assist in lipid absorption, including lipid soluble vitamins. The primary bile acids including cholic acid and chenodeoxycholic acid are converted to the secondary bile acids deoxycholic and lithocholic acids, respectively with the help of intestinal bacteria. Lithocholic acid (LCA) is one of the main secondary bile acids and possesses important biological properties. LCAs in aqueous media reach their critical micellar concentration (CMC) and typically self-assemble into micelles. Mostly CMC values range from 1 to 20 μM depending primarily on the medium's ionic strength are the main determinants of this value. These lithocholic acid-based micelles have the ability to dissolve certain lipids, including phospholipids and cholesterol, and develop mixed micelles in the bile. It is also important small molecule in enzyme science. For example, lithocholic acid conjugated with NAPE-PLD, a hydrolase enzyme, has been recently reported to enhance the hydrolysis rate Having combining self-assembly and catalytic ability features, LCA and LCA derivatives is considered to be a bioactive molecule. We checked its morphology and used it in hydrolase activity study. LCA derivative showed mild activity. To enhance the hydrolysis rate, we added metal ions to the solution, and interestingly, gelation occurred. The resulting metallogel was further characterized and used for enzymatic study and bacterial study. Our multifunctional lithocholic acid derivative was found to be catalytically active and exhibited probiotic activity beneficial for the growth of useful bacteria.

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