Cellobiose Regulator and Glucose Inducible System in Escherichia Coli

Das, Anshuman (2025) Cellobiose Regulator and Glucose Inducible System in Escherichia Coli. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Anshuman Das (20MS124)) 20MS124_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

For efficient breakdown of lignocellulosic biomass to simple sugars and for scaling up the production of biofuels, there has been profound progress in the development of novel engineered cellulases with low saccharification cost and high yield of downstream sugars. However, there has been a lack in the efficient screening system of these cellulases. Currently used methods are labour expensive, time consuming and tedious. This project aimed to develop a synthetic biosensor system using the promoter engineering technique and assembly methods. This system would sense the downstream products of the cellulases and will efficiently screen the activity without the need of extensive in-vitro assay systems. Once the cellulases start acting on the cellulosic biomass, it will produce oligosaccharides, cellobiose and glucose. Our sensing system would combined sense these sugars and give reporter protein signal accordingly. The cloning and pathway assembly was based on an adapted modular assembly method involving different assembly levels through the golden gate assembly method. This system will have various application starting from bioreactors in industries to other synergistic applications.

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