Mouse Hepatitis Virus (MHV) Infection Alters Expression of Cellular Prion Protein in Microglia/Macrophage

Ranawat, Nishtha (2014) Mouse Hepatitis Virus (MHV) Infection Alters Expression of Cellular Prion Protein in Microglia/Macrophage. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS dissertation of Nishtha Ranawat) Nishtha_Ranawat.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

Microglia, the Central Nervous System resident macrophages, are the principal resident immune cells of the brain. At the outset, immunofluorescence characterization of J774A.1, a macrophage cell line and N9, a microglia cell line was done using Cd11b, a membrane marker and Iba1, a cytosolic marker. Alongside these, characterization was also done for peritoneal macrophages and brain microglia isolated from C57BL/6 mice. To our surprise, although there was an increase in protein levels, PrPᶜ mRNA levels (absolute value) didn’t undergo marked difference across infected and uninfected cells. This needs to be confirmed by comparing cellular PrP mRNA levels in non-infected and infected, employing comparative Ct method using appropriate endogenous control along with test samples.

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