Study of Membrane Fluctuations and Cortex Thickness on Differentiating Myoblasts

Sivan, Athul M (2017) Study of Membrane Fluctuations and Cortex Thickness on Differentiating Myoblasts. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (PhD dissertation of Athul M Sivan (12MS074)) Master_Thesis_Athul_M_Sivan_12MS074.pdf - Submitted Version Restricted to Repository staff only Download (2MB)

Abstract

The C2C12 myoblast cells differentiate to myotubes under serum depleted conditions. During the myogenesis the cells align close to each other and then fuse. This results in the formation of myotubes. The differentiating C2C12 cells undergo various changes that could aid them in the fusion and adaptation to the muscular physiology. These changes range from cytoskeleton remodeling to major mechanical transduction pathways. Reports suggest that the rigorous changes in cytoskeleton transmit mechanical tension to plasma membrane. Changes in membrane tension could be addressed using the membrane fluctuation of cells. C2C12 being a cell line that undergo drastic mechanical changes during differentiation is a perfect system to study the spatio-temporal membrane fluctuations. The differentiating cells are subjected to major morphological changes. The cells fuse, they grow in size and they become elongated tubes. Cortical actin is one of the important cytoskeleton players who could regulate the morphology of the cell, which could have a great effect on myogenesis. So changes in actin cortex are something worth studying. Therefore in this study, we would to study membrane fluctuations and cortex thickness on differentiating myoblasts.

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