Multi-Residue Analysis of Pesticides in Plantation Crops and Vegetables

Doley, Jitu (2013) Multi-Residue Analysis of Pesticides in Plantation Crops and Vegetables. Masters thesis, Indian Institute of Science Education and Research Kolkata.

PDF (MS Dissertation of Jitu Doley (08MS47)) Jitu_Doley-08MS47-MSThesis.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

The objective of the study was to determine and compare the current level of exposure of the population to hazardous pesticide consumption of fresh vegetables produced in around Nadia district (Kancharapara, Jaguli and Barasat).Residual levels of pesticides were determined in 12 samples of 4 varieties of vegetables and plantation crops collected from selected sites around Nadia (Kancharapara, Jaguli and Barasat).The procedure involves initial single-phase extraction of 10 g sample with 10 ml acetonitrile, followed by liquid–liquid partitioning formed by addition of 4 g anhydrous mgso4 plus 1 g nacl. Removal of residual water and cleanup are performed simultaneously by using a rapid procedure called dispersive solid-phase extraction (dispersive-SPE), in which 150 mg anhydrous mgso4 and 25 mg primary secondary amine (PSA) sorbent are simply mixed with 1 ml acetonitrile extract. The dispersive- SPE with PSA Effectively removes many polar matrix components, such as organic acids, certain polar pigments, and sugars, to some extent from the food extracts. Gas chromatography/mass spectrometry (GC/MS) is then used for quantitative and confirmatory analysis of GC-amenable pesticides. The data obtained was compared with that of a referenced pesticide by matching molecular weight to a library of known pesticides. Factors studied were retention time, relative abundance, concentration and molecular weight. The results indicated that all the vegetable samples have levels of one or more pesticides in them. The concentration found in the pesticides was very high. Because many vegetables are consumed fresh or only slightly cooked, the study shows that intensive vegetable production, common in study site , threatens public health from the pesticide dimensions. Standard recommendations to address this situation (better legislations, law enforcement, or integrated pest management) often do not match the capabilities of farmers and authorities. The most appropriate entry point for risk decrease that also addresses post harvest contamination is washing vegetables before food preparation at the household.

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