The Scaling Of Nuclei Size During Early Zebrafish Development And In Response To Ploidy

Rana, Paresh Priyadarshan (2021) The Scaling Of Nuclei Size During Early Zebrafish Development And In Response To Ploidy. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Paresh Priyadarshan Rana (16MS055)) 16MS055_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

Understanding biological processes in animal models provide unique insights into animal development and pathological conditions. In addition to understanding the genetic and molecular mechanisms, animal models also provide insights into biophysical properties that shape organs and tissues. The role of cell size in triggering developmental events such as zygotic genome activation (ZGA) and gastrulation has been well-studied. The phenomenon of molecular regulation and cell size variations during development have similarities with pathological conditions. Cell migration during metastasis can be modelled using the movement of cells during gastrulation of animal embryos. Altered ribosomal DNA copy numbers in Schizophrenia could be modelled on the dynamically changing copy numbers of ribosomal DNA during animal development. Thus, developmental biology is a powerful tool to understand homeostasis and disease states. Nuclei sizes are known to alter in disease conditions. In my project, I explore how nuclei sizes evolve during early cell divisions in the zebrafish embryo. During this phase, we have already shown that cell sizes follow a power law reduction. It turns out that even nucleus volume is altered when ploidy is changed. Comparison of mean nucleus volume in each stage between different ploidy conditions revealed that even though the ploidy's absolute nucleus size is affected by the ploidy, the exponent of reduction almost remains the same in three different ploidies.

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