Assessing Morphology, Adhesion, and Migration in Early vs Late Passage Periodontal Ligament derived Mesenchymal Stem Cells

Das, Arpita (2025) Assessing Morphology, Adhesion, and Migration in Early vs Late Passage Periodontal Ligament derived Mesenchymal Stem Cells. Masters thesis, Indian Institute of Science Education and Research Kolkata.

Text (MS Dissertation of Arpita Das (20MS140)) 20MS140_Thesis_file.pdf - Submitted Version Restricted to Repository staff only Download (1MB)

Abstract

Adhesion and migration in stem cells play an important role in development, wound healing, and immune response. In this study, we compared the morphology, adhesion, and migration properties of PDL-MSCs between early and late passages. We initially observed altered cell surface area between early and late passages, prompting us to take a closer look at its adhesion properties. Recognizing the biphasic relationship between cellular adhesion and migration as reported in studies before, we conducted experiments to compare migration properties between early and late passages. To gain deeper insights, we also compared focal adhesion characteristics of both early and late passages. Additionally, we analyzed the expression of certain key extracellular matrix genes that are reported to be involved in adhesion and migration. Our findings highlight how the adhesion and migration properties of MSCs are significantly influenced by passaging in vitro.

Actions (login required)

View Item